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BP-DL023

Masson三色染色试剂盒(固绿法)

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  • 英文名:
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  • Sbjbio
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  • 储存条件:
  • 室温,避光,12个月
产品编号 销售价促销价 库存 数量 单位 加入购物车
BP-DL023-50ml ¥280.00元 5553 EA 加入购物车
BP-DL023-100ml ¥470.00元 准现货 EA 加入购物车

商品描述

文献引用

质检证书(COA)

相关商品

【货号】 BP-DL023

【规格】 7×50mL/7×100mL

【保存】 10~30避光12个月有效。

【产品组成】

 image.png 

【产品简介】

结缔组织狭义上是指其含有的三种纤维:胶原纤维、网状纤维、弹力纤维而胶原纤维collagenfiber是分布最广、含量最多的一种纤维。Masson三色染色又称马松染色是结缔组织染色中最经典的一种方法是胶原纤维染色权威而经典的技术方法。所谓三色染色通常是指染胞核和能选择性的显示胶原纤维和肌纤维。该法染色原理不阴离子染料分子的大小和组织的渗透有关:分子的大小由分子量来体现小分子量易穿透结构致密、渗透性低的组织;而大分子量则只能进入结构疏松的、渗透性高的组织。然而淡绿或苯胺蓝的分子量都很大因此Masson染色后肌纤维呈红色胶原纤维呈绿色淡绿或蓝色苯胺蓝),主要用于区分胶原纤维和肌纤维。染色稳定;分化时间短1~2s;色彩清晰鲜艳;适用范围广适宜于组织的石蜡切片、冰冻切片等染色;所染切片保存时间长且不易褪色。

【使用方法】 

1、切片常规脱蜡至水用配制好的Weigert铁苏木素染色5~10min

2、用酸性乙醇分化液分化水洗。

3、用Masson蓝化液返蓝水洗。

4、蒸馏水洗1min

5、丽春红品红染色液染色5~10min

6、在上述操作过程中按蒸馏水:弱酸溶液=2:1比例配制弱酸工作液用弱酸工作液洗1min

7、磷钼酸溶液洗1~2min

8、用配制好的弱酸工作液洗1min

9、直接入固绿染色液中染色1~2min

10、用配制好的弱酸工作液洗1min

1195%乙醇快速脱水。无水乙醇脱水3每次5~10s

12、二甲苯透明3每次1~2min。中性树胶封固。

染色结果

 image.png 

【注意事项】

1、切片脱蜡应尽量干净。固定起着重要的作用使用不同的固定液可延或缩短染色时间。

2、取A1A2等量混合即为Weigert铁苏木素染液一般24h失去染色力。

3、酸性乙醇分化时间应根据切片厚薄、组织的类别和新旧而定。

4、弱酸溶液可使色彩更清晰鲜艳如使用量大可自行配制0.1~0.3%乙酸溶液予以替代。

5、磷钼酸分化时要在镜下控制分化到胶原纤维呈淡红色、纤维呈红色即可。分化时间根据染色深浅而定一般1~2min

6Masson蓝化液亦可自行配制Scott促蓝液或0.1~1%碳酸锂水溶液予以替代。


[1]  Ma X, Ma X, Ma Z, et al. The effects of uygur herb Hyssopus officinalis L. on the process of airway remodeling in asthmatic mice[J]. Evidence-Based Complementary and Alternative Medicine, 2014, 2014.

[2]  Wang W, Hu J, He C, et al. Heparinized PLLA/PLCL nanofibrous scaffold for potential engineering of small‐diameter blood vessel: Tunable elasticity and anticoagulation property[J]. Journal of Biomedical Materials Research Part A, 2015, 103(5): 1784-1797.

[3]  Wu C, Dong S, Li Y. Effects of miRNA-455 on cardiac hypertrophy induced by pressure overload[J]. International journal of molecular medicine, 2015, 35(4): 893-900.

[4]  Ren Z, Wang Y, Ma S, et al. Effective bone regeneration using thermosensitive poly (N-isopropylacrylamide) grafted gelatin as injectable carrier for bone mesenchymal stem cells[J]. ACS applied materials & interfaces, 2015, 7(34): 19006-19015.

[5]  Duan S, Yang X, Mei F, et al. Enhanced osteogenic differentiation of mesenchymal stem cells on poly (l‐lactide) nanofibrous scaffolds containing carbon nanomaterials[J]. Journal of Biomedical Materials Research Part A, 2015, 103(4): 1424-1435.

[6]  Ruan J, Wang X, Yu Z, et al. Enhanced Physiochemical and Mechanical Performance of Chitosan‐Grafted Graphene Oxide for Superior Osteoinductivity[J]. Advanced Functional Materials, 2016, 26(7): 1085-1097.

[7]  Jin J, Lv R, Guo J, et al. Improvement of left ventricular remodelling by inhibition of NF-κB in a rat model of myocardial infarction[J]. Heart, Lung and Circulation, 2016, 25(10): 1007-1012.

[8]  Jia Y, Wang W, Zhou X, et al. Synthesis and characterization of poly (glycerol sebacate)-based elastomeric copolyesters for tissue engineering applications[J]. Polymer Chemistry, 2016, 7(14): 2553-2564.

[9]  Xiao T, Luo J, Wu Z, et al. Effects of hydrogen sulfide on myocardial fibrosis and PI3K/AKT1-regulated autophagy in diabetic rats[J]. Molecular medicine reports, 2016, 13(2): 1765-1773.

[10]  Chi Y C, Shi C L, Zhou M, et al. Selective cyclooxygenase-2 inhibitor NS-398 attenuates myocardial fibrosis in mice after myocardial infarction via Snail signaling pathway[J]. Eur Rev Med Pharmacol Sci, 2017, 21(24): 5805-5812.

[11]  Ren Z, Ma S, Jin L, et al. Repairing a bone defect with a three-dimensional cellular construct composed of a multi-layered cell sheet on electrospun mesh[J]. Biofabrication, 2017, 9(2): 025036.

[12]  Liang B, Xiao T, Long J, et al. Hydrogen sulfide alleviates myocardial fibrosis in mice with alcoholic cardiomyopathy by downregulating autophagy[J]. International journal of molecular medicine, 2017, 40(6): 1781-1791.

[13]  Zhang Z, Li Z, Cao K, et al. Adjunctive therapy with statins reduces residual albuminuria/proteinuria and provides further renoprotection by downregulating the angiotensin II–AT1 pathway in hypertensive nephropathy[J]. Journal of hypertension, 2017, 35(7): 1442-1456.

[14]  Gong Z, Tang J, Xiang T, et al. Genome‑wide identification of long noncoding RNAs in CCl4‑induced liver fibrosis via RNA sequencing[J]. Molecular medicine reports, 2018, 18(1): 299-307.

[15]  Hou H T, Wang Y N, Shao S Z, et al. High calcium diet alleviates 5/6 nephrectomy-induced bone deteriorations of lumbar vertebrae in mice[J]. Experimental and therapeutic medicine, 2018, 15(4): 3483-3488.

[16]  Tang B, Zhang J G, Tan H Y, et al. Astragaloside IV inhibits ventricular remodeling and improves fatty acid utilization in rats with chronic heart failure[J]. Bioscience reports, 2018, 38(3).

[17]  Liu R, Zhang H B, Yang J, et al. Curcumin alleviates isoproterenol-induced cardiac hypertrophy and fibrosis through inhibition of autophagy and activation of mTOR[J]. European review for medical and pharmacological sciences, 2018, 22(21): 7500-7508.

[18]  Gong Z, Lin J, Zheng J, et al. Dahuang Zhechong pill attenuates CCl4‐induced rat liver fibrosis via the PI3K‐Akt signaling pathway[J]. Journal of cellular biochemistry, 2019.

[19]  Zhang W X, Tai G J, Li X X, et al. Inhibition of neointima hyperplasia by the combined therapy of linagliptin and metformin via AMPK/Nox4 signaling in diabetic rats[J]. Free Radical Biology and Medicine, 2019, 143: 153-163.

[20]  Deng Y, Guo S, Wei B, et al. Activation of nicotinic acetylcholine α7 receptor attenuates progression of monocrotaline-induced pulmonary hypertension in Rats by downregulating the NLRP3 inflammasome[J]. Frontiers in Pharmacology, 2019, 10: 128.

[21]  Lin J, Deng C, Peng Y, et al. Dynamic Changes in MMP1 and TIMP1 in the Antifibrotic Process of Dahuang Zhechong Pill in Rats with Liver Fibrosis[J]. Open Chemistry, 2019, 17(1): 346-356.

[22]  Yuan Z, Wei P, Huang Y, et al. Injectable PLGA microspheres with tunable magnesium ion release for promoting bone regeneration[J]. Acta biomaterialia, 2019, 85: 294-309.

[23]  Wei P, Jing W, Yuan Z, et al. Vancomycin-and Strontium-Loaded Microspheres with Multifunctional Activities against Bacteria, in Angiogenesis, and in Osteogenesis for Enhancing Infected Bone Regeneration[J]. ACS applied materials & interfaces, 2019, 11(34): 30596-30609.

[24]  Wei P, Yuan Z, Jing W, et al. Strengthening the potential of biomineralized microspheres in enhancing osteogenesis via incorporating alendronate[J]. Chemical Engineering Journal, 2019, 368: 577-588.

[25]  Zhou C, Li C, Wang Q, et al. Histopathological and proteomic analyses identify integrin-β1 as a potential mediator of phlebosclerosis in uremic patients[J]. Clinical and Experimental Nephrology, 2019: 1-9.

[26]  Shi A, Heinayati A, Bao D, et al. Small molecule inhibitor of TGF-β signaling enables robust osteogenesis of autologous GMSCs to successfully repair minipig severe maxillofacial bone defects[J]. Stem cell research & therapy, 2019, 10(1): 172.successfully repair minipig severe maxillofacial bone defects[J]. Stem cell research & therapy, 2019, 10(1): 172.